Elias Hakalehto, Doc., Ph.D.
Department of Biosciences
University of Eastern Finland
P.O.B. 1627
FI-70211 Kuopio
Finland
Abstract, 41st R3-Symposium of the R3-Nordic Society in Espoo 25.-26.5.2010
The PMEU (Portable Microbe Enrichment Unit) technology (Samplion Oy, Siilinjärvi, Finland) is a microbe cultivation method producing advantageous growth conditions for individual bacterial cells. Therefore, monitoring of bacterial populations and the presence of potential contaminants is optimised in terms of speed and accuracy. Hygienically important isolates of various bacterial strains have been cultivated aerobically, microaerobically or anaerobically using standard broth media by standard PMEU enhanced enrichment technology, or by PMEU Spectrion ® or PMEU Scentrion ® sensored units equipped with optical, IR, or gas sensors. The units were produced by Samplion Oy according to the ISO 9001 accepted protocol. The reference cultivations were carried out using standard microbiological procedures. The PMEU Spectrion ® is being validated by the VTT of Finland. PMEU versions facilitated ultra-fast detection of coliformic bacteria, bacilli, salmonellas, staphylococci, streptococci, campylobacteria and other groups. The PMEU Scentrion ® equipped with gas sensors for volatile organic compounds detected the contaminants at concentrations of bacterial levels around 10-1000 cfu/ml in 2-5 hours. Also the hospital validation studies in Austria and Finland are summarized. Moreover, The PMEU has been demonstrated to help the environmentally stressed cells to recover and become viable in the enrichment cultures. Hygiene sampling was carried out using a specific sampling syringe serving also as an incubator in the PMEU.
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It is a most important task to detect the pathogens from patient samples and to construct an effective treatment so fast as possible.
ReplyDeleteIn addition to this rapid detection of harmful/hazardous microorganisms, quantitative analyses are also available by applying the Detection Time principle to PMEU Spectrion and PMEU Scentrion methods: Detection Time is inversely correlated to Colony Counts. This procedure benefits eg. the control of water hygiene and the rapid biocide testing in all process industry enterprises.